Isolation and characterization of six human hepatic isometallothioneins.

The Isolation and Characterization a Human Diploid Cell Strain and Its Use in Production of Measles Vaccine

ISOLATION AND CHARACTERIZATION OF SIX HIGHLY INTRINSIC FLUORESCENT METABOLITES IN ARMILLARIA MELLEA (VAHL. EX. FR.) KARST

Six fluorescent compounds were isolated and characterized in rhizomorph of A. mellea and were presented first as: F , F , F , MF , MF and MF . These compounds differed by Rf on chromatoplaque, max in UV spectra and ex, em and max in fluorescence spectra. Their GC-MS analysis indicated that F has a phenolic moiety and the other five are the benzenic derivatives. F compound was more f...

Isolation and in vitro Characterization of Mesenchymal Stem Cells Derived from the Pulp Tissue of Human Third Molar Tooth

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...

THE ISOLATION OF ENZYME TRANSKETOLASE FROM HUMAN ERYTHROCYTES: THE CHARACTERIZATION OF ITS QUARTERNARY STRUCTURE

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

Isolation and Characterization of Human Induced Pluripotent Stem Cells-Derived Mesenchymal Progenitors

Purpose: Isolating human induced pluripotent stem cells (hiPS)-derived mesenchymal progenitors as a new source of mesenchymal cells which can differentiate into different lineages like adipose and bone. Materials and Methods: After 7 days of hiPS1 culture on matrigle coated dishes, spindle like cells around colonies were removed by cell scraper. These cells that had mesenchymal like morphology ...